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If the columns all have the same type of data, then yes, you can use the dynamic input without headers. When you set up the dynamic input, make sure to check the First Row Contains Data box. Alteryx will give each column a name (F1, F2...etc).
It was giving some error in resolving with .xls and .xlsx files with that. Even with columns being same (they looked same to me), there were some errors. I am working around that by using a Batch macro to Union the files.